THESIS ON SCRUB TYPHUS

Scrub typhus being the re-emerging zoonosis is increasingly recognised in India. Author information Article notes Copyright and License information Disclaimer. Materials and Methods It is a prospective study which was carried out between January to June 3 years 6 months , in a tertiary care hospital in Puducherry, South India. The most common diagnostic test in India is the Weil-Felix test. Clinical diagnosis of scrub typhus is challenging as the signs and symptoms of scrub typhus are similar to other febrile illnesses. None Acknowledgement for financial support and specific contribution only: The IgM ELISA with good sensitivity and specificity, ease to perform, swift results and also suitable for testing large number of specimens may be considered as good replacement for Weil-Felix test and IFA test in diagnosis of scrub typhus.

Find articles by Suryanarayana Bettadpura Shamanna. This is hospital based study which has its own limitations, therefore future research can be carried out with community-based study to get more data on seroprevalance of scrub typhus in Pondicherry. Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to Orientia tsutsugamushi in Thailand. After screening patients with acute febrile illness with a battery of diagnostic tests such as Para F for malaria, Widal tests, ICT for dengue and Ig M ELISA for leptospirosis, 63 samples reacted positively for above mentioned tests and were excluded. The IFA slide presents antigens from only 3 serotypes namely Karp, Kato and Gilliam, thus unable to detect other antigenically variable strains of Orientia tsutsugamushi affecting the sensitivity of the test [ 14 ]. A preliminary report from Pondicherry.

This is hospital based study which has its own limitations, therefore future research can be carried out with community-based study to get more data on seroprevalance of scrub typhus in Pondicherry.

thesis on scrub typhus

The patients attending this hospital as both inpatients and outpatients who were clinically diagnosed to have typhus fever were included. In a study by Sharma et al. Int J Microbiol Res Rev. A study was conducted in Delhi among the febrile paediatric patients which did not have definitive diagnosis.

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Though the presence of eschar is helpful in making a diagnostic clue for scrub typhus it is not always present. Introduction Scrub typhus has been one of the most covert emerging and re-emerging Rickettsial infections with increasing trend in incidences of the disease worldwide including India.

Nested PCR for detection of thphus kDa gene specific for Orientia tsutsugamushi with good sensitivity of and specificity may be recommended in laboratories with molecular lab facility [ 14 ]. Published online Nov 1.

The clinical symptoms are fever, headache, myalgia, malaise, rash and lymphadenopathy which are commonly seen in other acute febrile illness like malaria, enteric fever, leptospirosis, dengue etc. Open in a separate window. Results After screening patients with acute febrile illness with a battery of diagnostic tests such as Para F for malaria, Widal tests, ICT for dengue and Ig M ELISA for leptospirosis, 63 samples reacted positively for above mentioned tests and were excluded.

In our study single time point estimation is done, which is a major drawback.

Serodiagnosis of Scrub Typhus at a Tertiary Care Hospital from Southern India

The drawback of serology test is that the advantage of testing directly the presence of organism is not possible [ 14 ]. The preferential site of mite bite where the eschar is thdsis mostly in thesie intertriginous surfaces axilla, scrotum, perianal region which can be easily overlooked by the doctors [ 16 ].

J Clin Diagn Res. In this study an attempt has been made to know the seropositivity in clinically suspected scrub typhus patients. Asian J Pharm Clin Res.

Find articles by Belgode Narasimha Harish. Scrub typhus is widespread, extending from Japan to Australia and from India to Pacific [ 2 ]. The current gold standard for serology is Indirect Immunofluorescence Antibody IFA test which uses a fluorescence labelled anti-human immunoglobulin to detect antibodies in patients serum that have bound to immobilised thesiis antigen that is coated on the IFA slide.

Indian J Med Microbiol. The IgM ELISA with good sensitivity and specificity, ease to perform, swift results and also suitable for testing large number of specimens may be considered as good replacement for Weil-Felix test and IFA test in xcrub of scrub typhus. J Assoc Physicians India. Ann Indian Acad Neurol.

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Serodiagnosis of Scrub Typhus at a Tertiary Care Hospital from Southern India

The study was conducted only after obtaining written informed consent from the patients. The patients who had a history of fever with or without eschar and rash and also had more than 2 symptoms such as head ache, myalgia, malaise, nausea, abdominal discomfort were included. All serological tests are retrospective where diagnosis typyus done at the end of one week.

First case of scrub typhus with meningoencephalitis from Kerala: The cut-off value was obtained by calculating the average of optical density OD plus three times of standard deviation SD from serum samples of healthy individuals.

The current gold thsis is imperfect as it is expensive requiring a fluorescence microscope.

thesis on scrub typhus

The test is widely in use as it is cheap, easily available and does not require technical expertise. Find articles by Madhan Sugumar. The medical fraternity needs to understand the pitfalls of currently available Weil-Felix test which is still common in India and must include tests with better performance like IgM ELISA and nested PCR in the diagnostic algorithm of scrub typhus to reduce the burden of the disease.

In developing countries, among the various laboratory tests to diagnose scrub typhus, Weil-Felix test is commonly performed despite its low sensitivity. Scrub typhus, a zoonotic disease is one of the most covert emerging and re-emerging Rickettsial infections.

High-level expression of a kilodalton protein gene bor56 of Rickettsia tsutsugamushi Boryong and scdub application to enzyme-linked immunosorbent assays.